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METHODS GLP-1RAs decrease the cholesterol level and regulate the expression of cholesterol metabolism-associated genes in HepG2 cells treated with PA In HepG2 cells, treatment with PA significantly increased cholesterol levels in both cell lysates and culture supernatants
The ability of probenecid to inhibit cAMP accumulation in GLP-1(2836)treated human caECs further supports our finding that GLP-1(2836) works through sAC to increase intracellular cAMP levels (Supplemental Figure 8C)
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