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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)
doi: 10.1016/j.cmet.2017.09.019 11 BoulangC
If youre unsure, consider rotating between the abdomen and thigh to see which feels more comfortable and provides better results
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